This test method covers a protocol for capturing, preserving and purifying nucleic acids and quantifying total bacterial content in fuel and fuel-associated water using quantitative polymerase chain reaction.
qPCR; quantitative polymerase chain reaction; bacteria; fuel; microbiology
There is no standard for qPCR in fuel - several years ago a standard practice was published but lacks specific methodological details. qPCR is a more commonly used method for microbial detection and quantification and the industry would benefit from a standard method.
The title and scope are in draft form and are under development within this ASTM Committee.
Date Initiated: 07-17-2024
Technical Contact: Jordan Schmidt