Work Item
ASTM WK91650

New Test Method for the measurement of total bacteria in fuel and fuel associated water using quantitative polymerase chain reaction (qPCR)

1. Scope

This test method covers a protocol for capturing, preserving and purifying nucleic acids and quantifying total bacterial content in fuel and fuel-associated water using quantitative polymerase chain reaction.

Keywords

qPCR; quantitative polymerase chain reaction; bacteria; fuel; microbiology

Rationale

There is no standard for qPCR in fuel - several years ago a standard practice was published but lacks specific methodological details. qPCR is a more commonly used method for microbial detection and quantification and the industry would benefit from a standard method.

The title and scope are in draft form and are under development within this ASTM Committee.

Details

Developed by Subcommittee: D02.14

Committee: D02

Staff Manager: Alyson Fick

Work Item Status

Date Initiated: 07-17-2024

Technical Contact: Jordan Schmidt